Evidence library

Independent trials guiding our frass recommendations.

Explore ten peer-reviewed studies plus five on-the-ground programs that document how BSFL frass performs in vegetables, turf, tree nurseries, and modern CEA systems.

Peer-reviewed evidence

What the journals say about BSFL frass.

The library below tracks ten experiments that quantify yield, nitrogen efficiency, microbiome shifts, and tree-restoration outcomes, plus five industry programs translating the science into practice.

Peer-reviewed agronomy & soil science

These open-access papers span field cereals, greenhouse herbs, hemp, tree nurseries, and soil ecology. Click through for full methods and statistics.

Wageningen University PhD, 2023

Cabbage root fly suppression

Wantulla showed that BSF frass and exuviae only suppressed Delia radicum when they primed resident Pseudomonas communities; efficacy and larval mortality were soil-type specific yet achieved without compromising cabbage biomass.

Read dissertation

bioRxiv, 2023

Wheat growth despite low NPK

Green et al. found winter wheat shoots were 11% taller and heavier after frass drenches even though the material contained only trace N:P:K. Enterococcus species were traced from larval guts into the rhizosphere as the likely plant-growth trigger.

Read study

Insects (MDPI), 2021

HexaFrass™ greenhouse trials

Borkent & Hodge measured up to 25% more shoot dry weight across herbs and vegetables when 3 g/pot of BSF frass was mixed into organic media, matching chicken manure pellets but stalling at higher doses.

Read study

Heliyon, 2021

Ryegrass biomass response

Menino et al. ran seven BSF frass rates and saw total ryegrass fresh weight climb from 42 g/pot in the control to 62 g/pot at 150 kg N ha−1, alongside higher soil P, K, and dehydrogenase activity.

Read study

J. Insects as Food & Feed, 2022

Maize yield + profit lift

Treating greenhouse maize with BSF frass boosted grain yield by 2–25% over NPK or brewer’s grain compost and raised net income 163% when frass was paired 50:50 with mineral N.

Read study

Frontiers in Plant Science, 2020

Field-ready nitrogen efficiency

Beesigamukama et al. reported that 7.5 t/ha of BSF frass produced 14% more maize grain than a commercial organic fertiliser and 7% more than urea, while increasing N uptake by 76% and doubling agronomic N-use efficiency.

Read study

Agronomy (MDPI), 2020

Soil hygiene & nitrate supply

Klammsteiner et al. showed frass matched NH4NO3 for ryegrass production, increased nitrate and dissolved N, and did not elevate coliform counts after soil incorporation.

Read study

Frontiers in Microbiology, 2022

Microbiome + respiration gains

Fuhrmann et al. tracked Rwandan grass–clover pots and saw BSF residues lift plant yield 17% and basal respiration 16% over controls while enriching Bacillus and Mortierella populations.

Read study

Sustainability (MDPI), 2025

Reforestation-ready seedlings

Malagasy trials found half- and single-rate BSF frass doubled Dodonaea height growth and kept survival at 95%, whereas NPK cut survival to 5%; overdosing (2× rate) was lethal, underscoring the need for calibrated applications.

Read study

Insects (MDPI), 2025

Hemp cultivar response

Kavanagh et al. evaluated six monoecious and dioecious hemp cultivars and found low HexaFrass™ doses (3 g/pot) increased shoot growth in both male and female plants with no marginal gains at higher rates, mirroring other organic fertilisers.

Read study

Industry + field deployments

Programs below show how BSFL frass is being commercialised in North America and Africa—from broiler feed safety to urban microgreen substrates and tree nurseries.

  • Enterra Natural Fertilizer field program (2013) — Three British Columbia trials found 5 t/ha of frass delivered commercial bok choi, lettuce, and potato yields while 5–8% (dw/dw) starter mixes outperformed worm castings and hinted at potential wireworm suppression.
  • EnviroFlight & Southern Poultry Feed (Modern Poultry, 2024) — Broiler diets containing 2.5% starter, 5% grower, and 10% finisher frass maintained bodyweight and feed conversion, validating the material as a safe poultry ingredient.
  • IITA BBEST processing hubs (Ghana, 2024) — Three BSF facilities were commissioned to recycle urban bio-waste into larvae feed and frass for chicken, fish, and vegetable cooperatives within a 50 km supply radius.
  • IITA Agrifair deployment (Ghana, 2025) — PPRDS-certified frass was showcased alongside live BSF demos, prompting prison systems and agrifood investors to request training so they can add frass production to local programs.
  • SARE ONE24-435 urban microgreens (USA, 2024–2026) — Boston Microgreens, Apogee Farm, and UDC are replacing peat with BSF frass for microgreens and vegetable starts while documenting germination, yield, and irrigation adjustments for other urban growers.

Trial snapshots

Using the evidence in real programs.

Translate the studies above into clear agronomy talking points for crews, retailers, or funding partners.

Row crops & cereals

Field maize responded best to 2.5–7.5 t/ha of frass, with the 7.5 t/ha rate adding 14% grain and +76% N uptake over urea; pairing lower rates 1:1 with mineral N kept ROI positive.

Controlled environments

Greenhouse herbs, lettuce, and hemp all peaked when 3 g/pot (≈5% of media) of HexaFrass™ was blended into peat mixes; higher doses plateaued or suppressed growth, so keep additions light.

Tree nurseries

Malagasy reforestation nurseries doubled Dodonaea height growth and held 95% survival with half- and single-rate frass (0.325–0.65 g N/plant); synthetic NPK dropped survival to 5%.

Soil ecology & composting

BSF residues increased basal respiration 16%, plant yield 17%, and encouraged Bacillus/Mortierella populations while matched NH4NO3 for ryegrass yields—evidence that frass feeds microbes before plants.

Downloads & links

Keep learning and cite with confidence.

These resources pair well with the studies above when pitching clients or writing grant proposals.

Need a custom trial protocol?

We collaborate with universities and private labs to design plots, capture microbial data, and translate findings into SOPs.